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anti-myosin heavy chain  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank anti-myosin heavy chain
    Anti Myosin Heavy Chain, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+myosin+heavy+chain/anti-Myosin+heavy+chain/custom%40f1%2E652%4042810481
    Average 96 stars, based on 325 article reviews
    anti-myosin heavy chain - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Validation of an in vitro muscle platform to evaluate myogenesis and calcium handling in control and dystrophic human myotubes.
    Article Snippet: Protein samples were diluted to 0.5 μg/μL with 5X Fluorescence Master Mix (Biotechne) and denatured at 95 °C for 5 minutes.

    Article Title: Dietary combination of sucrose and linoleic acid increases intramyocellular lipid and impairs muscle strength in female Zucker diabetic fatty rats
    Article Snippet: solution (Polysciences, Warrington, PA) for antigen retrieval for 30 min, permeabilized with 0.1% Triton X-100 for 20 min, and blocked for 1 h in blocking solution (1 × PBS, 1.5% goat serum, 0.1% Triton X-100).

    Saline:

    Article Title: The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity.
    Article Snippet: .. Larvae were sequentially rehydrated, washed in 0.1% Triton X-100 in 1× phosphate buffered saline (PBST), blocked in 5% horse serum in PBST for 4 h and incubated in primary antibody solution [antiCOL2A1 (Developmental Studies Hybridoma Bank, II-II6B3, mouse, 1:50); anti-THBS4 (Abcam, ab211143, rabbit, 1:500); anti-GFP (Abcam, ab13970, chicken, 1:500); anti-sox9a (Abcam, ab209820, rabbit, 1:500); anti-col10a1 (Developmental Studies Hybridoma Bank, mouse X-AC9, 1:50); anti-col1 (Abcam, ab23730, rabbit, 1:100); anti-myosin heavy chain (Developmental Studies Hybridoma Bank, A4.1025, mouse, 1:100)] overnight at 4°C with gentle agitation. .. Following this, larvae were washed in PBST, re-blocked for 2 h and incubated in secondary antibody solution (AlexaFluor donkey anti-mouse 488, AlexaFluor donkey anti-rabbit 568, both 1:500) and/or WGA conjugate (CF640R WGA Biotium, 2 μg/ml) overnight at 4°C with gentle agitation.

    Article Title: The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity
    Article Snippet: .. Larvae were sequentially rehydrated, washed in 0.1% Triton X-100 in 1× phosphate buffered saline (PBST), blocked in 5% horse serum in PBST for 4 h and incubated in primary antibody solution [anti-COL2A1 (Developmental Studies Hybridoma Bank, II-II6B3, mouse, 1:50); anti-THBS4 (Abcam, ab211143, rabbit, 1:500); anti-GFP (Abcam, ab13970, chicken, 1:500); anti-sox9a (Abcam, ab209820, rabbit, 1:500); anti-col10a1 (Developmental Studies Hybridoma Bank, mouse X-AC9, 1:50); anti-col1 (Abcam, ab23730, rabbit, 1:100); anti-myosin heavy chain (Developmental Studies Hybridoma Bank, A4.1025, mouse, 1:100)] overnight at 4°C with gentle agitation. .. Following this, larvae were washed in PBST, re-blocked for 2 h and incubated in secondary antibody solution (AlexaFluor donkey anti-mouse 488, AlexaFluor donkey anti-rabbit 568, both 1:500) and/or WGA conjugate (CF ® 640R WGA Biotium, 2 μg/ml) overnight at 4°C with gentle agitation.

    Incubation:

    Article Title: The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity.
    Article Snippet: .. Larvae were sequentially rehydrated, washed in 0.1% Triton X-100 in 1× phosphate buffered saline (PBST), blocked in 5% horse serum in PBST for 4 h and incubated in primary antibody solution [antiCOL2A1 (Developmental Studies Hybridoma Bank, II-II6B3, mouse, 1:50); anti-THBS4 (Abcam, ab211143, rabbit, 1:500); anti-GFP (Abcam, ab13970, chicken, 1:500); anti-sox9a (Abcam, ab209820, rabbit, 1:500); anti-col10a1 (Developmental Studies Hybridoma Bank, mouse X-AC9, 1:50); anti-col1 (Abcam, ab23730, rabbit, 1:100); anti-myosin heavy chain (Developmental Studies Hybridoma Bank, A4.1025, mouse, 1:100)] overnight at 4°C with gentle agitation. .. Following this, larvae were washed in PBST, re-blocked for 2 h and incubated in secondary antibody solution (AlexaFluor donkey anti-mouse 488, AlexaFluor donkey anti-rabbit 568, both 1:500) and/or WGA conjugate (CF640R WGA Biotium, 2 μg/ml) overnight at 4°C with gentle agitation.

    Article Title: The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity
    Article Snippet: .. Larvae were sequentially rehydrated, washed in 0.1% Triton X-100 in 1× phosphate buffered saline (PBST), blocked in 5% horse serum in PBST for 4 h and incubated in primary antibody solution [anti-COL2A1 (Developmental Studies Hybridoma Bank, II-II6B3, mouse, 1:50); anti-THBS4 (Abcam, ab211143, rabbit, 1:500); anti-GFP (Abcam, ab13970, chicken, 1:500); anti-sox9a (Abcam, ab209820, rabbit, 1:500); anti-col10a1 (Developmental Studies Hybridoma Bank, mouse X-AC9, 1:50); anti-col1 (Abcam, ab23730, rabbit, 1:100); anti-myosin heavy chain (Developmental Studies Hybridoma Bank, A4.1025, mouse, 1:100)] overnight at 4°C with gentle agitation. .. Following this, larvae were washed in PBST, re-blocked for 2 h and incubated in secondary antibody solution (AlexaFluor donkey anti-mouse 488, AlexaFluor donkey anti-rabbit 568, both 1:500) and/or WGA conjugate (CF ® 640R WGA Biotium, 2 μg/ml) overnight at 4°C with gentle agitation.

    Article Title: The Effects of Creatine Monohydrate and/or Whey Protein on the Muscle Protein Synthesis and Anabolic Signaling Responses in Non-Stressed C2C12 Murine Myotubes
    Article Snippet: .. Cells were then blocked with PBS/Triton containing 1% BSA for 1 h at room temperature, followed by incubation with a primary antibody solution containing anti-myosin heavy chain (1:100) (DSHB, Iowa City, IA, USA; Cat. No. A4.1025) in PBS/Triton/BSA for 3 h at room temperature. .. Following 3 × 3 min washes with PBS/Triton, cells were incubated with a secondary antibody solution containing goat anti-mouse IgG2a AF488 (Thermo Scientific; Cat. No. A-21131) in PBS/0.2% Triton-X for 2 h at room temperature.

    Gentle:

    Article Title: The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity.
    Article Snippet: .. Larvae were sequentially rehydrated, washed in 0.1% Triton X-100 in 1× phosphate buffered saline (PBST), blocked in 5% horse serum in PBST for 4 h and incubated in primary antibody solution [antiCOL2A1 (Developmental Studies Hybridoma Bank, II-II6B3, mouse, 1:50); anti-THBS4 (Abcam, ab211143, rabbit, 1:500); anti-GFP (Abcam, ab13970, chicken, 1:500); anti-sox9a (Abcam, ab209820, rabbit, 1:500); anti-col10a1 (Developmental Studies Hybridoma Bank, mouse X-AC9, 1:50); anti-col1 (Abcam, ab23730, rabbit, 1:100); anti-myosin heavy chain (Developmental Studies Hybridoma Bank, A4.1025, mouse, 1:100)] overnight at 4°C with gentle agitation. .. Following this, larvae were washed in PBST, re-blocked for 2 h and incubated in secondary antibody solution (AlexaFluor donkey anti-mouse 488, AlexaFluor donkey anti-rabbit 568, both 1:500) and/or WGA conjugate (CF640R WGA Biotium, 2 μg/ml) overnight at 4°C with gentle agitation.

    Article Title: The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity
    Article Snippet: .. Larvae were sequentially rehydrated, washed in 0.1% Triton X-100 in 1× phosphate buffered saline (PBST), blocked in 5% horse serum in PBST for 4 h and incubated in primary antibody solution [anti-COL2A1 (Developmental Studies Hybridoma Bank, II-II6B3, mouse, 1:50); anti-THBS4 (Abcam, ab211143, rabbit, 1:500); anti-GFP (Abcam, ab13970, chicken, 1:500); anti-sox9a (Abcam, ab209820, rabbit, 1:500); anti-col10a1 (Developmental Studies Hybridoma Bank, mouse X-AC9, 1:50); anti-col1 (Abcam, ab23730, rabbit, 1:100); anti-myosin heavy chain (Developmental Studies Hybridoma Bank, A4.1025, mouse, 1:100)] overnight at 4°C with gentle agitation. .. Following this, larvae were washed in PBST, re-blocked for 2 h and incubated in secondary antibody solution (AlexaFluor donkey anti-mouse 488, AlexaFluor donkey anti-rabbit 568, both 1:500) and/or WGA conjugate (CF ® 640R WGA Biotium, 2 μg/ml) overnight at 4°C with gentle agitation.



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